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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
24/04/1998 |
Data da última atualização: |
13/07/2023 |
Autoria: |
OLIVEIRA, S. A. de; MACHADO, M. de F. P. da S.; PRIOLI, A. J.; MANGOLIN, C. A. |
Título: |
In vitro propagation of Cereus Peruvianus mill. (Cactaceae). |
Ano de publicação: |
1995 |
Fonte/Imprenta: |
In Vitro Cell. Development Biology, v. 31, n. 1, p. 47-50, 1995. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Cereus peruvianus seedlings were used as a source of stem explants to determine the effective conditions for inducing and maintaining callus tissues in a state of rapid growth, as well as to obtain plants regenerated from callus cultures. Factorial combinations of 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin in MS medium were tested, and we concluded that the 18.1 uM 2,4-D and 18.6 or 27.9 1M kinetin combinations were suitable for callus induction. The cactus shoots were produced from the friable callus; root elongation occurred within 2 wk in medium without 2,4-D and with 18.6 1iM kinetin. This method can be used to rapidly produce many C. peruvianus plan. |
Palavras-Chave: |
Cactacea; Friable callus; Seedling stem explants; Shoot formation; Succulent plants. |
Thesagro: |
Cactaceae; Cacto. |
Thesaurus Nal: |
In vitro culture; Micropropagation. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
Marc: |
LEADER 01436naa a2200265 a 4500 001 1523885 005 2023-07-13 008 1995 bl uuuu u00u1 u #d 100 1 $aOLIVEIRA, S. A. de 245 $aIn vitro propagation of Cereus Peruvianus mill. (Cactaceae).$h[electronic resource] 260 $c1995 520 $aAbstract: Cereus peruvianus seedlings were used as a source of stem explants to determine the effective conditions for inducing and maintaining callus tissues in a state of rapid growth, as well as to obtain plants regenerated from callus cultures. Factorial combinations of 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin in MS medium were tested, and we concluded that the 18.1 uM 2,4-D and 18.6 or 27.9 1M kinetin combinations were suitable for callus induction. The cactus shoots were produced from the friable callus; root elongation occurred within 2 wk in medium without 2,4-D and with 18.6 1iM kinetin. This method can be used to rapidly produce many C. peruvianus plan. 650 $aIn vitro culture 650 $aMicropropagation 650 $aCactaceae 650 $aCacto 653 $aCactacea 653 $aFriable callus 653 $aSeedling stem explants 653 $aShoot formation 653 $aSucculent plants 700 1 $aMACHADO, M. de F. P. da S. 700 1 $aPRIOLI, A. J. 700 1 $aMANGOLIN, C. A. 773 $tIn Vitro Cell. Development Biology$gv. 31, n. 1, p. 47-50, 1995.
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